Resources

Published Research Using Xanapath Technology

• Huebschman ML, Rosenblatt KP, and Garner HR. Hyperspectral microscopy imaging to analyze pathology samples with multi-colors reduces time and cost. Proceedings of SPIE Volume 7182: Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 2009.Hyperspectral microscope imaging system used to scan and analyze pathology tissue samples stained with 4 standard fluorochromes attached to specific antibodies. Quantitative values are recorded and can be compared to the visual interpretations.
• Geho, D., Lahar, N., Gurnani, P., Huebschman, M., Herrmann, P., Espina, V., Shi, A., Wulfkule, J., Garner, H., Petricoin III, E.F., Liotta, L.A., and Rosenblatt, K.P.  Pegylated, Steptavidin-Conjugated Quantum Dots Are Effective Detection Elements for Reverse-Phase Protein Microarrays. Bioconjugate Chem. 2005;16:559-566.Hyperspectral imaging using a quantum dot reporter on a reverse phase protein microarray. Demonstrated unamplified detection of signaling proteins, and potential for multiplexed, high-throughput reverse-phase protein microarrays in which numerous analytes are measured in parallel within a single spot.
• O’Brien KM et al. ASTRAL, a hyperspectral imaging DNA sequencer. Rev. Sci. Instrum. 1998; 69( 5):2141-46.Prototype DNA sequencer using hyperspectrographic separation and detection of the fluorescence emission to image gel.
• Schultz RA et al. Hyperspectral Imaging: A Novel Approach For Microscopic Analysis. Cytometry. 2001;43:239–247.Hyperspectral imaging microscope tested with samples relevant to cytogenetic, histologic, cell fusion, microarray scanning, and materials science applications.
• Huebschman ML, Schultz RA and Garner HR. Characteristics and Capabilities of the Hyperspectral Imaging Microscope Single-Pass Multispectral Imaging Enables Applications Ranging from Whole-Chromosome Painting to Gene Mapping. IEEE Engineering In Medicine and Biology. 2002;104-117.Technical description of “Hyperspectral Imaging Microscope Workstation,” including hardware and software components, and capabilities to collect and correlate 10 fluorochromes on a single pass without use of optical filters.
• Huebschman ML et al. Design and performance of a variable spectrum synthesizer. Rev. Sci. Instrum. 2004;75:4845-4855.Describes a variable spectrum synthesizer capable of synthesizing multiple spectra with varying intensities and wavelengths; application to phototherapy for skin cancer presented.
• Geho, G.H., Killian, J.K., Nandi, A., Pastor, J., Gurnani, P., and Rosenblatt, K.P. (2007)  Fluorescence-based analysis of cellular protein lysate arrays using quantum dots.  Methods in Molecular Biology 374: 229-238.
• Huebschman, M.L., Rosenblatt, K.P., and Garner, H.R. (2009) Hyperspectral microscopy imaging to analyze pathology samples with multi-colors reduces time and cost. Proceedings of SPIE, BiOS 2009, in press.
• Rosenblatt, K.P., Michael L. Huebschman, M.L., and Garner, H.R. (2009) Construction and Hyperspectral Imaging of Quantum Dot Lysate Arrays. Methods in Molecular Biology, in press.

Presentations

• Hyperspectral microscopy imaging to analyze pathology samples with multi-colors reduces time and cost. Michael L. Huebschman, Kevin P. Rosenblatt, and Harold R. Garner Presented Michael Huebschman at SPIE Photonics West, San Jose, California, January 24-29, 2009.
• Liu H. “Color Makes Life Better – A New Pathological Method for Molecular Profiling of Cancer,” December 16, 2008, University of Texas Southwestern Medical Center, Cancer Immunobiology Center, Works-in-Progress.

Further Reading

• http://en.wikipedia.org/wiki/Hyperspectral_imaging
• http://rst.gsfc.nasa.gov/Intro/Part2_24.html